By Ben Dawes (ed.)
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Extra resources for Advances in Parasitology, Vol. 3
In EATRO, 1962-63), but it is clearly desirable to avoid unnecessary addition of foreign, particularly antigenic, substances. (c) The preserved material should be altered as little as possible from its original condition by the treatment before preservation. Manipulation should be carried through as quickly as possible and as continuously as possible at 0" C. (d) If the trypanosomes for preservation are to be suspended in fluids other than those of the body of their host a medium of experimentallyestablished suitability is necessary.
He arranged for G. palpalis to take their first blood meal from a host infected with T. gambiense and studied the infection rates occurring in flies fed in this way on the lst, 2nd, and subsequent, days after eclosion. 1 and 0%. Wijers did not allow any substitute meal to the flies which were not allowed to feed on the infected host on the 1st and 2nd days and so his result might have been related to some starvation effect. However, van Hoof et al. (1937) did allow a substitute meal and their results were similar.
Smaller individuals emerged from the pupa with less fat than did larger ones and this appeared to be because the amount of fat laid down at the beginning of pupal development was relatively small in small individuals. Estimates were made of the size which a fly must be if it is to complete its development and live to obtain its first blood meal. Comparisons of these estimates with the size distribution of two species in cold and hot seasons suggested that exhaustion of the pupal fat reserves might play a part in limiting the distribution under extreme climatic conditions (Bursell, 1960b).
Advances in Parasitology, Vol. 3 by Ben Dawes (ed.)